Bme Paper Osteoblast Maturation

In: Science

Submitted By megcmcd
Words 8554
Pages 35
JBMR

ORIGINAL ARTICLE

Osteoblast Maturation and New Bone Formation in
Response to Titanium Implant Surface Features Are
Reduced With Age
Rene Olivares-Navarrete, 1 * Andrew L Raines, 1,2 * Sharon L Hyzy, 1 Jung Hwa Park, 1 Daphne L Hutton, 1
David L Cochran , 3 Barbara D Boyan , 1 and Zvi Schwartz 1,3
1

Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA
St. Joseph’s Translational Research Institute, Atlanta, GA, USA
3
University of Texas Health Science Center at San Antonio, San Antonio, TX, USA
2

ABSTRACT
The surface properties of materials contribute to host cellular response and play a significant role in determining the overall success or failure of an implanted biomaterial. Rough titanium (Ti) surface microtopography and high surface free energy have been shown to enhance osteoblast maturation in vitro and increase bone formation in vivo. Whereas the surface properties of Ti are known to affect osteoblast response, host bone quality also plays a significant role in determining successful osseointegration. One factor affecting host bone quality is patient age. We examined both in vitro and in vivo whether response to Ti surface features was affected by animal age.
Calvarial osteoblasts isolated from 1-, 3-, and 11-month-old rats all displayed a reduction in cell number and increases in alkaline phosphatase–specific activity and osteocalcin in response to increasing Ti surface microtopography and surface energy. Further, osteoblasts from the three ages examined displayed increased production of osteocalcin and local factors osteoprotegerin, vascular endothelial growth factor (VEGF)-A, and active transforming growth factor (TGF)-b1 in response to increasing Ti surface roughness and surface energy. Latent TGF-b1 only increased in cultures of osteoblasts from 1- and 3-month-old…...

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