Microbiology Unknown Bacteria

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Submitted By schoolgirl
Words 1873
Pages 8
INTRODUCTIONS:
Microorganisms are both beneficial and harmful. These microorganisms are important to humans because they play a role in the ecology of life, by decomposing wastes, both natural and man-made, such as creating nitrogen fertilizer at the root zones of certain crops. Other several pathogens that can cause serious harm, even immediate death due to the diseases or disease causing products they produce. Overall, microorganisms play an important role in life.
The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics. The differential tests used to identify the unknown cultures were Gram staining, oxidase, indole test, urea test, and casein test.
MATERIALS AND METHODS:

The unknown bacteria were given out by the lab instructor. Each student chose their own unknown bacteria according to the number. All methods have been practiced to ensure proper procedure identifying bacteria have been applied to this unknown. Procedures were followed as stated in the course laboratory manual provided by the instructor, unless otherwise noted. Each test performed identified was used to determine the specifics and identify the unknown bacterium. All of the following tests were performed on this unknown on February 09, 2008. Some of the tests required a follow-up right after the next lab.
The first procedure that needed to be accomplished was to streak the unknown out on a Trypticase Soy Agar plate, using the T streak method described in the lab manual. The unknown culture was inoculated in the Tryptic Soy Broth tube, a TSA plate agar, and a TSA slant. This procedure is needed to be done in order to test…...

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